Short communication, Note
https://doi.org/10.2478/10004-1254-62-2011-2076
The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts
Ana Bogović
; University of Zagreb School of Dental Medicine, Zagreb, Croatia
Jana Nižetić
; University of Zagreb School of Dental Medicine, Zagreb, Croatia
Nives Galić
; University of Zagreb School of Dental Medicine, Zagreb, Croatia
Davor Želježić
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Vedran Micek
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Marin Mladinić
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Abstract
The aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. The rats were killed and extracted teeth sectioned transversely through the pulp. The slices were placed in a RPMI 1640 cell culture medium supplemented with 10 % foetal calf serum. During 14 days of cultivation cultures were treated with preparations that contained hyaluronic acid (Gengigel Prof®), and calcium hydroxide (ApexCal®), or with dentin adhesive (Excite®). Cellularity and viability of fibroblasts and odontoblasts was analysed using a haemocytometer. Hyaluronic acid proved most efficient and the least toxic for direct pulp capping. Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility.
Keywords
direct pulp capping; fibroblast proliferation; odontoblastic proliferation
Hrčak ID:
69302
URI
Publication date:
17.6.2011.
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