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Veterinary Archives, Vol. 81 No. 3, 2011.

Original scientific paper

Identification and characterization of immunodominant antigen(s) of Toxocara vitulorum in buffaloes (Bubalus bubalis).

Jyoti ; Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Nirbhay K. Singh ; Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Prayag D. Juyal ; Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India

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Abstract

Electrophoretic analysis of perienteric fluid antigen (PeAg), uterine antigen (UtAg), body wall antigen (BWAg) and whole worm extract antigen (WWEAg) showed 26, 23, 20 and 21 polypeptides in the range of 11.5-147.3, 20.3-147.3, 19.7-139.5 and 20.3- 126.3 kDa, respectively. Among the 4 antigens, 7 polypeptides of 20.3, 43.6, 53.1, 57.4, 66.9, 111.3 and 126.3 kDa were found to be common. The Western blotting analysis of PeAg, UtAg, BWAg and WWEAg, using high titre sera from heavily infected buffalo calves, revealed 5 immunodominant polypeptides of the mol. wt. 11.5, 66.9, 74.9, 105.5 and 126.3 kDa; 4 polypeptides of the mol. wt. 66.9, 83.4, 105.5 and 111.3 kDa; 6 polypeptides of 24.1, 33.7, 43.6, 57.4, 60.3 and 76.2 kDa; and 4 polypeptides of 30.4, 53.1, 57.4 and 66.9 kDa, respectively. The 66.9 kDa antigen was found to be immunodominant in 3 of the referral antigens (PeAg, UtAg and WWEAg) while 105.5 kDa antigen was immunodominant in PeAg and UtAg and a 57.4 kDa antigen in BWAg and WWEAg. Cross reactivity of Haemonchus spp. and Paramphistomum epiclitum antigens with T. vitulorum positive sera by western blotting revealed no cross reactive polypeptide and the 66.9 kDa antigen found in the somatic antigens of T. vitulorum may be a specifi c immunodominant antigen and could be further exploited for immunodiagnosis of latent toxocarosis.

Keywords

buffalo; immunodiagnosis; somatic antigens; Toxocara vitulorum; Western blotting

Hrčak ID:

69487

URI

https://hrcak.srce.hr/69487

Publication date:

20.6.2011.

Article data in other languages: croatian

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