Veterinary Archives, Vol. 81 No. 4, 2011.
Original scientific paper
Isolation, identification and molecular characterization of Mycoplasma isolates from goats of Gujarat State, India.
Pankaj Kumar
; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand, Anand Agricultural University, Gujarat, India
Ashish Roy
; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand, Anand Agricultural University, Gujarat, India
Bharat B. Bhanderi
; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand, Anand Agricultural University, Gujarat, India
Bhik C. Pal
; Department of Veterinary Epidemiology, Veterinary University, Mathura (U. P.), India
Abstract
A total of 358 samples [nasal discharges (215), lung tissue (6), pleural fluid (4), joint fluid (5), milk (60), preputial swabs (22), conjunctival swabs (16), ear swabs (5) and vaginal discharges (25)] were subjected for cultural isolation on MBHS-L and MBHS-A media. Of these, a total of 30 isolates (8.35 %) of Mycoplasma species, (20 from nasal discharges, 4 from lung tissue, 2 from pleural fluid, 3 from milk and 1 from joint fluid) were isolated and characterized biochemically and identified as M. mycoides subsp. capri and M. capricolum subsp. capricolum by PCR and PCR-RFLP. The isolates were identified as the Mycoplasma species by their cultural, morphological, biochemical characteristics and this was further confirmed by PCR and PCR-RFLP using different cluster, group and species specific primer pairs and by restriction enzyme respectively. This is a rare report on the isolation, identification and molecular characterization of goat Mycoplasma species in Western India. These studies have revealed a high prevalence of Mycoplasma species infection in Western India.
Keywords
India; mycoplasmas; goats; M. mycoides subsp. capri; M. capricolum subsp. capricolum
Hrčak ID:
70931
URI
Publication date:
20.7.2011.
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