Original scientific paper

https://doi.org/10.5562/cca1822

Electrokinetic Study of Bovine Serum Albumin Adsorption on Previously Formed PAH/PSS Multilayer

Irena Đapić ; Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
Davor Kovačević orcid.org/0000-0001-6446-3459 ; Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia

Abstract

Layer-by-layer structures (e.g. polyelectrolyte multilayers and protein-polyelectrolyte multilayers)
play a very important role in surface modification processes. In the present study, electrokinetic measurements
were applied for the investigation of poly(allylamine hydrochloride)/poly(sodium
4-styrenesulphonate) (PAH/PSS) multilayer formation, with PAH being a terminal layer, as a function of
pH. Additionally, the effect of supporting electrolyte (KCl) concentration on multilayer formation was
tested. Silica particles were used as the solid substrate. Furthermore, the adsorption of bovine serum albumin
(BSA) on previously formed multilayer was examined as a function of pH and BSA concentration.
It was confirmed that the electrokinetic measurements are suitable for monitoring the formation of various
multilayers. In all investigated systems the process of multilayer formation was found to depend on conditions
(ionic strength and pH) under which the multilayer was formed. Moreover, in the case of BSA, the
adsorption on previously formed multilayer BSA concentration also plays a significant role. (doi:
10.5562/cca1822)

Keywords

polyelectrolyte multilayers; electrokinetics; adsorption; bovine serum albumin; ionic strength

Hrčak ID:

71981

URI

https://hrcak.srce.hr/71981

Publication date:

3.10.2011.

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