Original scientific paper

Histopathological evaluation and immunohistochemical study of estrogen receptor α, HER-2 and Ki-67 in canine neoplastic mammary lesions.

Andrea Gudan Kurilj ; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Marko Hohšteter ; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Branka Artuković ; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Krešimir Severin ; Department of Forensic Veterinary Medicine, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivan-Conrado Šoštarić-Zuckermann ; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ana Beck ; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sven Seiwerth ; Institute of Pathology, School of Medicine, University of Zagreb, Zagreb, Croatia
Ruža Sabočanec ; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Željko Grabarević ; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

The frequency of different types of mammary lesions and their relationship with histologic grade was investigated. One hundred and forty-six mammary lesions were classified according to the World Health Organization (WHO) criteria. Selected lesions (51 malignant and 24 benign) were investigated to determine the immunohistochemical expression of estrogen receptor alpha (ERα), HER-2 and Ki-67 and their relationship with tumor factors. The most common breeds affected were cross breeds, poodles, cocker spaniels and German shepherds and the median age of tumor diagnosis was 10 years (range 4 - 15 years of age). Classification of all canine mammary gland lesions revealed 110 (75.3%) malignant and 36 (24.7%) benign tumors. ERα was expressed by 9/24 (37.5%) benign and 9/51 (17.6%) malignant tumors. HER-2 protein was detected in 5/51 (9.8%) malignant tumors but none in the 24 benign tumors. The Ki-67 index was higher in malignant (mean 29.5) than benign (mean 9.1) tumors (P<0.001) and significantly higher in anaplastic carcinomas than tubulopapillary carcinomas (P<0.05).

Keywords

mammary tumors; dog; ERα; HER-2; Ki-67

Hrčak ID:

74653

URI

https://hrcak.srce.hr/74653

Publication date:

5.12.2011.

Article data in other languages: croatian

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