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Acta Pharmaceutica, Vol. 62 No. 1, 2012.

Original scientific paper

https://doi.org/10.2478/v10007-012-0008-z

Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells

ZOHREH MOHAMMADI ; Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
FARID ABEDIN DORKOOSH ; Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
SAMAN HOSSEINKHANI ; Department of Biochemistry, Faculty of Science, Tarbiat Modares University, Tehran, Iran
TINA AMINI ; Nobleceuticals Ltd, Birmingham, United Kingdom
AMIR ABBAS RAHIMI ; Nanobiotechnology Department, Pasteur Institute of Iran, Tehran, Iran
ABDOLHOSSEIN ROUHOLAMINI NAJAFABADI ; Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
MORTEZA RAFIEE TEHRANI ; Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

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Abstract

A successful gene delivery system requires efficiency and stability during storage. Stability studies are imperative for nanomedicines containing biotechnological products such as plasmids and targeting peptides. Chitosan-DNA-FAP-B nanoparticles are novel non-viral vectors for specific gene delivery to the lung epithelial cells. In this study, the storage stability of chitosan-DNA-FAP-B nanoparticles at –20, 5 and 24 ºC was examined. Size, zeta potential and transfection efficiency of these nanoparticles in storage were also evaluated.
Stability studies showed that chitosan-DNA-FAP-B nanoparticles were stable after 1 month when stored at –20 °C and retained their initial size, zeta potential and transfection efficiency. However, their stability was not desirable at 5 and 24 ºC. Based on these results, it can be concluded that chitosan-DNA-FAP-B nanoparticles can be a promising candidate for gene delivery to lung epithelial cells with good storage stability at –20 ºC during 1 month.

Keywords

chitosan; FAP-B; nanoparticle; stability; gene delivery

Hrčak ID:

76603

URI

https://hrcak.srce.hr/76603

Publication date:

31.3.2012.

Article data in other languages: croatian

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