Original scientific paper

Doubtful and discordant results in fluorescent antibody test for rabies diagnosing.

Peter Hostnik ; Institute for Microbiology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia
Marjeta Štrancar ; Institute for Microbiology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia
Darja Barlič-Maganja ; Institute for Microbiology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia
Jože Grom ; Institute for Microbiology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia

Abstract

A total of 7339 fox, dog, cat, cattle, sheep and other mammalian brains were tested to rabies virus antigen. Results of fluorescent antibody test (FAT) were evaluated by two microscopists. Doubtful and discordant results in FAT were analyzed again in virus isolation test (VIT) using mouse neuroblastoma (NA) cell line. Twenty-eight brain samples were determined as doubtful, while 9 brain samples were determined to be positive by one microscopist and negative by the other. Samples which were shown as doubtful and discordant in FAT were retested in VIT. Seventeen of these were positive in the VIT.

Keywords

rabies; immunofluorescence; cell culture

Hrčak ID:

84684

URI

https://hrcak.srce.hr/84684

Publication date:

21.4.2001.

Article data in other languages: croatian

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