Original scientific paper
The Cytotoxic Effect of Sodium Hypochlorite on Cell Cultures
Ivana Miletić
; Department of Dental Pathology, School of Dental Medicine University of Zagreb, Gundulićeva 5, 10000 Zagreb, Croatia
Alena Knežević
; Department of Dental Pathology, School of Dental Medicine University of Zagreb, Gundulićeva 5, 10000 Zagreb, Croatia
Ivica Anić
; Department of Dental Pathology, School of Dental Medicine University of Zagreb, Gundulićeva 5, 10000 Zagreb, Croatia
Maja Osmak
; Department of Molecular Genetics, Ruder Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
Jozo Šutalo
; Department of Dental Pathology, School of Dental Medicine University of Zagreb, Gundulićeva 5, 10000 Zagreb, Croatia
Zita Blažić-Potočki
; Stomatological polyclinic, Perkovčeva 3, 10000 Zagreb, Croatia
Abstract
Sodium hypochlorite (NaOCl) is the most popular irrigation solution used in root canal treatment, in spite of the fact that it may irritate the vital tissues. This study was designed to evaluate the cytotoxic effect of a water solution of sodium hypochlorite on cultures of human cervical carcinoma HeLa cells and mouse L929 fibroblasts by determining the number of vital cells. The tested concentrations of 0.5%, 1.0%, 2.5%, 5.25% were prepared in Eagle's growth medium. The number of cells was counted by electronic counter while the number of viable cells was determined, after the addition of nigrosin dye, by a light microscope. Concentration of 0.5% NaOCl caused the least changes in morphology, number and percentage of viable cells while 5.25% concentration caused total loss of HeLa and L929 cells. All tested concentrations were cytotoxic with significant difference (p<0.05) compared to the control group. However, the results showed that there was no significant difference between 1.0% and 2.5% concentration of NaOCl for both cell lines (p<0.05).
Keywords
sodium hypochlorite; cell culture; cytotoxicity
Hrčak ID:
94757
URI
Publication date:
15.3.1999.
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