Original scientific paper
https://doi.org/10.15644/asc47/2/4
Antimicrobial Effectiveness of Intracanal Ozone Treatment
Bernard Janković
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Eva Klarić
orcid.org/0000-0003-3447-5517
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Katica Prskalo
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Danijela Marović
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Vlatko Pandurić
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Zrinka Tarle
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Abstract
Aim: The aim of this study was to determine the amount of aerobic, anaerobic, and total number of bacterial colonies after chemomechanical root canal treatment and after additional intracanal
disinfection using ozone. Materials and Methods: Twenty - three patients with the same number of single rooted teeth (n=23) with periapical lesion (<5 mm) observed on retroalveolar radiogram or panoramic radiograph took part in this study. For the microbiological analysis three swabs were taken: immediately before (B1), after mechanical instrumentation and irrigation with 2.5% sodium hypochlorite (B2) and after chemomechanical treatment and additional intracanal ozone application (B3). Swabs were taken with sterile paper points, bacteria were cultivated and the results were interpreted after 14 days using the API 20A system. The data were analyzed by Wilcoxon Signed Rank Test measuring the number of bacterial colonies before and after the root canal treatment. Results: The difference between groups B1 and B2, B1 and B3 and B2 and B3 for all three groups: aerobic bacteria, anaerobic bacteria and total number of bacteria was statistically significant (p<0.05). Conclusion: Both methods showed a strong effect in bacterial number reduction.After the chemomechanical and additional ozone root canal treatment, the number of aerobic and anaerobic bacterial colonies further decreased compared to the measurements immediately before and after the chemomechanical root canal treatment. Because of the improvements in bacterial reduction after additional application of ozone in the root canal, this procedure is recommended for use in clinical practice.
Keywords
Anti-bacterial Agents; Root Canal Preparation; Ozone; Disinfection
Hrčak ID:
104980
URI
Publication date:
4.7.2013.
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