Original scientific paper
Tooth germ transplantation in rats : III Immunological activity of the parental strainon the tooth germ graft of F1 hybrid
Dragica Zaklan-Kavić
; Zavod za animalnu fiziologiju Prirodoslovno-matematičkog fakulteta, Zagreb, Jugoslavija
Andrija Kaštelan
; Zavod za animalnu fiziologiju Prirodoslovno-matematičkog fakulteta, Zagreb, Jugoslavija
Ivo Miše
; Zavod za oralnu kirurgiju Stomatološkog fakulteta, Zagreb, Jugoslavija
Abstract
The purpose of this paper is to study allograft immune reaction using the model of tooth germ transplantation between highly inbred strain Of rats, where rejection proceeds at a relatively uniform rate. The experiments were carried out in inbred rats of the Y59 and VM strain combinations differing in their major histocompatibility system (AgB). A strong unilateral histocompatibility barrier was made using Fi hybrid grafts to a parental strain. The donors of ithe tooth germs were 10-day old rats, and recipients were adult female rats. Germs of the first and second molar tooth dissected from the upper and lower jaw were transplanted into the subcutaneous tissue of the axilla, and beneath the renal Capsula. The grafts were removed at different intervals from 3 to 200 days after transplantation and examined histologically. The transplants were evaluated according to infiltration of the grafts with mononuclear cells, and the viability and further development of the tooth germs. The results revealed that the grafts showed various signs of rejection at each of the experimental period. Further development of the tooth germs was seriously impaired or completely arrested.
Keywords
Hrčak ID:
106781
URI
Publication date:
15.9.1975.
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