Original scientific paper

https://doi.org/10.2478/acph-2013-0036

Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD

JIANPING CHEN ; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China
ZHONGGUI LI ; School of Health and Pharmacy, Guandong Lingnan Institute of Technology, Guangzhou, China
KEN Y. Z. ZHENG ; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China
AVA J. Y. GUO ; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China
KEVIN Y. ZHU ; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China
WENDY L. ZHANG ; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China
JANIS Y. X. ZHAN ; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China
TINA T. X. DONG ; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China
ZIREN SU ; School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, China
KARL W. K. TSIM ; Division of Life Science and Centre for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China

Abstract

Gleditsiae Fructus Abnormalis and Gleditsiae Sinensis Fructus are obtained from different developmental stages of fruits from Gleditsia sinensis Lam. (Fam. Leguminosae). The possible interchangeable usage of the two fruits, however, has long been very controversial. Here, high performance liquid chromatography coupled with diode array detection was developed to explore their chemical fingerprinting profiles. Besides, the amounts of aglycons of saponin compounds, echinocystic acid and oleanolic acid in both fruits were quantified. The results indicated that there was no significant difference in the amount of aglycons from the two types of fruits; however, their chromatographic fingerprints showed distinct characteristics. Therefore, the interchangeable application of these fruits has to be taken with a specific precaution.

Keywords

gleditsiae fructus abnormalis; gleditsiae sinensis fructus; HPLC-DAD; fingerprinting

Hrčak ID:

110458

URI

https://hrcak.srce.hr/110458

Publication date:

31.12.2013.

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