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Archives of Industrial Hygiene and Toxicology, Vol. 65 No. 1, 2014.

Original scientific paper

https://doi.org/10.2478/10004-1254-65-2014-2416

3-nitrotyrosine levels in dichlorvos-induced neurotoxicity

Dilek Guvenç orcid id orcid.org/0000-0003-0036-0914 ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey
Abdurrahman Aksoy orcid id orcid.org/0000-0001-9486-312X ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey
Yavuz Kursad Das orcid id orcid.org/0000-0001-8634-5752 ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey
Enes Atmaca ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey
Oguzhan Yavuz ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey

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Abstract

The aim of this study was to evaluate dichlorvos toxicity in terms of nitro-oxidative stress by determining 3-nitrotyrosine (3-NT) levels in the fore, mid, and hindbrain regions in acutely exposed rats. Male Sprague- Dawley rats were randomly allocated to three groups of eight. Group 1 was administered a single intraperitoneal dichlorvos dose of 1.8 mg kg-1 (0.1xLD50) and group 2 a dose of 9 mg kg-1 (0.5xLD50). The control group received 0.5 mL saline solution via the same route. 3-NT and tyrosine (TYR) levels were measured using high performance liquid chromatography with a photodiode array detector (HPLC-PDA) and expressed as a ratio of 3-NT to TYR. The 3-NT/1000 TYR ratios increased significantly in the fore-, mid- and hindbrains of the exposed groups compared to control (p<0.01). In the forebrain, the increase was also significant between the treated groups. Our study has confirmed that acute exposure to dichlorvos leads to nitro-oxidative stress in the brain and that 3-NT may play a role in the mechanism of dichlorvos neurotoxicity.

Keywords

brain; HPLC; nitro-oxidative stress; pesticides; rats

Hrčak ID:

117136

URI

https://hrcak.srce.hr/117136

Publication date:

10.3.2014.

Article data in other languages: croatian

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