Original scientific paper
POLYMORPHISM OF PRLR-GENE IN BLACK SLAVONIAN SOWS: PRELIMINARY RESULTS
Antun Brkić
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska (student)
Sven Menčik
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Eleonora Bačani
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska (student)
Anamaria Ekert Kabalin
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Abstract
Population of Black Slavonian pig, once highly valued and almost the only breed in Slavonia, has significantly decreased over the years. With modest demands of nutrition and housing, they are intendend for the breeding in the open. This breed belongs in the middle fertile breed with 6-8 piglets per litter. It is characterized by high quality meat, meant for processing in traditional dried meat products. Research and preservation of this unique genetic potential are priority because of its quality. The aim of this preliminary study was to isolate DNA from hair follicle samples of 15 Black Slavonian sows, to genotype polymorphism of PRLR receptor and evaluate its possible connection with reproductive traits. This could contribute to improvement of selection which previously was based on phenotypic indicators. After DNA isolation and multiplication of PRLR sequence, obtained PCR products were subjected to restriction enzyme AluI in order to determine the polymorphism of PRLR-gene. The results have shown even distribution of AA (40%) and BB (40%) homozygotes, while AB heterozygote was less frequent (20%). The largest number of total born and live born piglets was in AB genotype, although the BB homozygotes observed greater variability in litter size. Despite the determined differences in litter size between genotypes they were not statistically significant.
Keywords
Black Slavonian pig; PRLR-gene; PCR; AluI; DNA
Hrčak ID:
138656
URI
Publication date:
2.4.2015.
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