Professional paper
Characterization of wool dust extract in vitro
Eugenija Žuškin
Jadranka Mustajbegović
Abstract
Wool dust has been described as a cause of respiratory impairment in workers occupationally exposed in wool processing. A pharmacological investigation was performed on 30 samples of isolated guinea pig trachea with wool dust water extracts prepared from the material collected at the workplaces of exposed workers. Wool extract was added into the system with Krebs solution in progressively increased concentrations of 10, 30, 100, 300 and 1000 fit. The constriction of the tracheal smooth musde caused by wool dust extract was diminished after pretreatment with pyrilamine (antihistamine) and verapamil (intracellular and extracellular calcium blocking agent). Atropine (anticholinergic) and indomethacin (inhibitor of prostaglandin synthesis) had very little impact on the bronchoconstrictive effect of wool dust extract on the tracheal smooth muscle. This finding suggests that the airway constriction induced by wool dust is partly attributable to mediators like histamine and also to calcium.
Keywords
pharmacological investigation; exposure to wool dust; isolated guinea pig trachea; respiratory disorders
Hrčak ID:
144667
URI
Publication date:
2.4.1997.
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