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Archives of Industrial Hygiene and Toxicology, Vol. 66 No. 3, 2015.

Original scientific paper

In vitro cytotoxicity evaluation of different pulp capping materials: a comparative study

Claudio Poggio orcid id orcid.org/0000-0002-9606-6930 ; Department of Clinical-Surgical, Diagnostic and Pediatric Sciences-Section of Dentistry, University of Pavia, Pavia, Italy
Matteo Ceci ; Department of Clinical-Surgical, Diagnostic and Pediatric Sciences-Section of Dentistry, University of Pavia, Pavia, Italy
Alberto Dagna ; Department of Clinical-Surgical, Diagnostic and Pediatric Sciences-Section of Dentistry, University of Pavia, Pavia, Italy
Riccardo Beltrami ; Department of Brain and Behavioral Sciences, University of Pavia, Pavia, Italy
Marco Colombo ; Department of Clinical-Surgical, Diagnostic and Pediatric Sciences-Section of Dentistry, University of Pavia, Pavia, Italy
Marco Chiesa ; Department of Clinical-Surgical, Diagnostic and Pediatric Sciences-Section of Dentistry, University of Pavia, Pavia, Italy

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Abstract

Direct pulp capping covers the exposed surface of the pulp to maintain its vitality and preserve its functional and biologic activity. The aim of the present study was to compare the biocompatibility effects of seven different pulp-capping materials in vitro: Dycal®, Calcicur®, Calcimol LC®, TheraCal LC®, ProRoot MTA®, MTA-Angelus®, and Biodentine®. Using the Transwell insert methodology by Alamar blue test, we evaluated the cytocompatibility of the above mentioned materials towards murine odontoblasts cells (MDPC-23) at three different times (24, 48, and 72 h). For additional control, the cell viability at 72 hours was also assessed by MTT assay. Morphological analysis of murine odontoblasts was assessed by Confocal Laser Scanning Microscope. The results indicate significantly different biocompatibility among materials with different composition. Biodentine® and mineral trioxide aggregate (MTA)-based products showed lower cytotoxicity, varying from calcium hydroxide-based materials, which exhibited higher cytotoxicity. Although our findings are limited to in vitro conditions, the observation that Biodentine® caused a cytotoxic effect similar to MTA suggests that it may be considered an alternative in pulp-capping treatment, as calcium hydroxide-based materials present higher cytotoxic effects.

Keywords

Alamar blue test; biocompatibility; dental material; MTT test; murine odontoblast

Hrčak ID:

145614

URI

https://hrcak.srce.hr/145614

Publication date:

24.9.2015.

Article data in other languages: croatian

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