Original scientific paper
https://doi.org/10.15255/CABEQ.2014.2037
Isolation, Fermentation Optimization and Performance Studies of a Novel Biosurfactant Producing Strain Bacillus amyloliquefaciens
W. Zhang
orcid.org/0000-0002-2834-3035
; College of Environment and Chemistry Engineering, Yanshan University, Qinhuangdao 066004, China
X. Zhang
; College of Environment and Chemistry Engineering, Yanshan University, Qinhuangdao 066004, China
H. Cui
; College of Environment and Chemistry Engineering, Yanshan University, Qinhuangdao 066004, China
Abstract
In this research, biosurfactant-producing bacteria were isolated from the outlet sludge of a canteen and one promising strain was identified through 16S rDNA sequence as Bacillus amyloliquefaciens. This strain can utilize water-soluble carbon source and the FT-IR analysis indicated the biosurfactant was probably glycolipids. Further factors (fermentation time, temperature, carbon source, nitrogen source, ion concentration) affecting the biosurfactant production were determined. The optimum fermentation conditions and nutrient concentration were as follows: fermentation time 48 h, temperature 30 °C, fermentation pH 6, glucose concentration 30 g L–1, peptone concentration 4 g L–1, total phosphorus concentration 8 g L–1, sodium chloride concentration 2 g L–1, magnesium sulfate concentration 0.25 g L–1, and calcium chloride concentration 0.04 g L–1. In such
conditions, the surface tension of the solution was 28 mN m–1. At the same time, the formation of biosurfactant and nano-silica complex system was discussed to improve the biosurfactant performance and provide the basis for expanding the application scope of biosurfactant.
Keywords
biosurfactant; Bacillus amyloliquefaciens; nano-silica
Hrčak ID:
147010
URI
Publication date:
14.10.2015.
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