Original scientific paper

The toxicity of isoproterenol to myocardial tissue in experimental conditions

P. Ljubunčić ; Institut za fiziologiju i biokemiju, Medicinski fakultet, Sarajevo, Bosna i Hercegovina
F. Mujić ; Institut za fiziologiju i biokemiju, Medicinski fakultet, Sarajevo, Bosna i Hercegovina
M. Winterhalter ; Institut za fiziologiju i biokemiju, Medicinski fakultet, Sarajevo, Bosna i Hercegovina
N. Huković ; Institut za fiziologiju i biokemiju, Medicinski fakultet, Sarajevo, Bosna i Hercegovina
Z. Vidović ; Institut za fiziologiju i biokemiju, Medicinski fakultet, Sarajevo, Bosna i Hercegovina
S. Đuričić ; Institut za fiziologiju i biokemiju, Medicinski fakultet, Sarajevo, Bosna i Hercegovina

Abstract

The effect of toxic doses of isoproterenol (ISP) on total activities and isoenzyme patterns of creatine kinase (CK) and lactate dehydrogenase (LOH) in rat sera was investigated and correlated with histopathological changes in the myocardial tissue. A single dose of 25 mg ISP per 100 g body weight caused a statistically important elevation of CK and LOH total activities and of the activities of cardiospecific isoenzymes CK-MB, LOH-1 and LOH-2 six hours after intraperitoneal administration. The isomorphic LOH isoenzyme pattern, which was also observed, was taken to be a proof of the ISP-induced cardiogenic shock. The increase in the activity of CK-MM and LOH-5 isoenzymes could be explained as a secondary consequence of cardiogenic shock and of the consecutive damage of the tissue with anaerobic metabolism such as liver. The findings were confirmed by a histopathological analysis showing the development of coagulative necrosis and myocytolysis as well as undulations of heart muscle cells as a sign of cardiogenic shock.

Keywords

serum enzymes in rat; synthetic catecholamine; toxic dose

Hrčak ID:

147842

URI

https://hrcak.srce.hr/147842

Publication date:

9.6.1992.

Article data in other languages: croatian

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