Short communication, Note
Ispitivanje biosinteze T-2 toksina u čistoj kulturi u različitim laboratorijskim uslovima
A. Bočarov-Stančić
; Institut za biološka istraživanja "Siniša Stanković" Beograd , Srbija
M. Muntanola-Cvetković
; Institut za biološka istraživanja "Siniša Stanković" Beograd , Srbija
Abstract
Seven isolates of the fungal genus Fusarium, comprising three species: F.culmorum, F.sporotrichioides and F.tricinctum, were tested for T-2 toxin production. Cultivation was carried oout concurrently on a natural substrate (wheat grains) under temperature stress conditions (7 days at 28 °C, 21 days at 15-16 °C), and in a semi-synthetic liquid medium (5% glucose, 0.1 % peptone-1, 0.1 % yeast extract) on a rotary shaker (200 rpm, 5 days at 26 °C). Of the fungal isolates cultivated on wheat grains 85.7% were found to be toxicogenic; the yields of T-2 toxin were rather small (12.5-52.5 mg/kg dry weight). In the liquid medium all Fusarium cultures biosynthesized T-2 toxin; the quantities produced ranged from 0.8 to 175.0 mg/l of the filtrate. At least half of the dispensed sugar was present in the medium when T-2 toxin production started. No correlation was found between the type of mycelial growth, sporulation, biomass quantities and the intensity of T-2 toxin biosynthesis. Most of the produced trichothecene was of extracellular origin. Fermentation in the semi-synthetic liquid medium using a rotary shaker was more suitable for screening the toxicity of the fungal isolates in pure cultures because of a shorter period of incubation, simpler sample preparation and T-2 toxin extraction. In this way factors influencing toxin production could be defined more precisely.
Keywords
Hrčak ID:
152849
URI
Publication date:
31.1.1989.
Visits: 1.281 *