Short communication, Note

https://doi.org/10.1515/acph-2016-0015

Preliminary in vitro evaluation of the anti-proliferative activity of guanylhydrazone derivatives

PAULO H. B. FRANÇA ; Laboratory of Medicinal Chemistry, School of Nursing and Pharmacy, Federal University of Alagoas, Maceio, Brazil
EDEILDO F. DA SILVA-JÚNIOR ; Laboratory of Medicinal Chemistry, School of Nursing and Pharmacy, Federal University of Alagoas, Maceio, Brazil
PEDRO G. V. AQUINO ; Natural Resources Research Laboratory, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceio, Brazil
ANTÔNIO E. G. SANTANA ; Natural Resources Research Laboratory, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceio, Brazil
JAMYLLE N. S. FERRO ; Cell Biology Laboratory, Biological and Health Sciences, Federal University of Alagoas, Maceio, Brazil
EMILIANO DE OLIVEIRA BARRETO ; Cell Biology Laboratory, Biological and Health Sciences, Federal University of Alagoas, Maceio, Brazil
CLÁUDIA DO Ó PESSOA ; Experimental Oncology Laboratory, Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, Brazil
ASSUERO SILVA MEIRA ; Experimental Oncology Laboratory, Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, Brazil
THIAGO M. DE AQUINO ; Laboratory of Medicinal Chemistry, School of Nursing and Pharmacy, Federal University of Alagoas, Maceio, Brazil
MAGNA S. ALEXANDRE-MOREIRA ; Institute of Biological and Health Sciences, Laboratory of Pharmacology and Immunity, Federal University of Alagoas, Maceio, Brazil
MARTINE SCHMITT ; Laboratoire d’innovation Thérapeutique, Faculté de Pharmacie, Université de Strasbourg, Illkirck, France
JOÃO X. DE ARAÚJO-JÚNIOR ; Laboratory of Medicinal Chemistry, School of Nursing and Pharmacy, Federal University of Alagoas, Maceio, Brazil

Abstract

Guanylhydrazones have shown promising antitumor activity in preclinical tumor models in several studies. In this study, we aimed at evaluating the cytotoxic effect of a series of synthetic guanylhydrazones. Different human tumor cell lines, including HCT-8 (colon carcinoma), MDA-MB-435 (melanoma) and SF-295 (glioblastoma) were continuous exposed to guanylhydrazone derivatives for 72 hours and growth inhibition of tumor cell lines and macrophages J774 was measured using tetrazolium salt (MTT) assay. Compounds 7, 11, 16 and 17 showed strong cytotoxic activity with IC50 values lower than 10 μmol L–1 against four tumor cell lines. Among them, 7 was less toxic to non-tumor cells. Finally, the obtained data suggest that guanylhydrazones may be regarded as potential lead compounds for the design of novel anticancer agents.

Keywords

guanylhydrazone; cytotoxic effect; synthesis; MTT assay

Hrčak ID:

153334

URI

https://hrcak.srce.hr/153334

Publication date:

31.3.2016.

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