Acta clinica Croatica, Vol. 40 No. 2, 2001.
Original scientific paper
Differences in the Antigenic Structure and Production of Hemolysin of Pseudomonas Aeruginosa Strains Isolated From a Variety of Isolation Sites
Jasmina Vraneš
Ivana Brkić
Mira Horonitz
Abstract
Pseudomonas (P.) aeruginosa produces a number of exoenzymes, which have been implicated as virulence factors in infections caused by this organism. One of these exoenzymes is phospholipase C or heat labile hemolysin (HE) or P. aeruginosa. The aim of the study was to establish differences in HE production of 100 P. aeruginosa strains in dependence of their antigenic structure and type of specimens from which they were isolated. Isolates were serotyped by agglutination with commercial antisera according to the International Antigenic Typing System. A microtiter HE assay was used to quantitate the hemolytic activity of the strains investigated. The most commonly detected O-serogroup was O11, found in 34% of strains, followed by O6 (16%), O3 (9%), O4 (8%), O2 (7%) and O1 (6%) serogroups. Serogroups O7, O10, O13, O14, O15 and O17 were not detected. Serogroup O11 was found in 11 out of 16 strains isolated from bronchial secretion, and in 2 of 15 strains isolated from urine (p<0.01). HE production was detected in 88% of all isolates. The O1, O2, O3 and O16 strains mostly produced a large quantity of HE, while the epidemic O11 strains produced a small amount of HE. Urine and pharyngeal isolates produced the highest levels of HE.
Keywords
Pseudomonas aeruginosa, pathogenicity; Pseudomonas aeruginosa, classification; Hemolysins
Hrčak ID:
14854
URI
Publication date:
1.6.2001.
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