Original scientific paper
Impact of Silver Nanoparticles on Haemolysis, Platelet Function and Coagulation
Julie Laloy
; Department of Pharmacy, Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences (NARILIS), NAmur MEdicine & Drug Innovation Center (NAMEDIC), Namur Thrombosis and Hemostasis Center (NTHC), University of Namur, Namu, Belgium
Valentine Minet
; Department of Pharmacy, Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences (NARILIS), NAmur MEdicine & Drug Innovation Center (NAMEDIC), Namur Thrombosis and Hemostasis Center (NTHC), University of Namur, Namu, Belgium
Lutfiye Alpan
; Department of Pharmacy, Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences (NARILIS), NAmur MEdicine & Drug Innovation Center (NAMEDIC), Namur Thrombosis and Hemostasis Center (NTHC), University of Namur, Namu, Belgium
François Mullier
; Department of Pharmacy, Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences (NARILIS), NAmur MEdicine & Drug Innovation Center (NAMEDIC), Namur Thrombosis and Hemostasis Center (NTHC), University of Namur, Namu, Belgium; Hematology D
Sonja Beken
; Division Evaluators, DG PRE Authorisation, Federal Agency for Medicines and Health Products (FAMHP), Brussels, Belgium
Olivier Toussaint
; Laboratory of Cellular Biochemistry and Biology, NNC, NARILIS, University of Namur, Namur, Belgium
Stéphane Lucas
; Research Centre for the Physics of Matter and Radiation, NNC, NARILIS, University of Namur, Namur, Belgium
Jean-Michel Dogné
; Department of Pharmacy, Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences (NARILIS), NAmur MEdicine & Drug Innovation Center (NAMEDIC), Namur Thrombosis and Hemostasis Center (NTHC), University of Namur, Namu, Belgium
Abstract
Silver nanoparticles (Ag NPs) are increasingly used in biomedical applications because of their large antimicrobial spectrum. Data in the literature on the ability of Ag NPs to perform their desired function without eliciting undesirable effects on blood elements are very limited and contradictory. We studied the impact of Ag NPs on erythrocyte integrity, platelet function and blood coagulation. Erythrocyte integrity was assessed by spectrophotometric measurement of haemoglobin release. Platelet adhesion and aggregation was determined by light transmission aggregometry and scanning electron microscopy. The calibrated thrombin generation test was used to study the impact on coagulation cascade. We demonstrated that Ag NPs induced haemolysis. They also increase platelet adhesion without having any impact on platelet aggregation. Finally, they also had procoagulant potential. Bringing all data from these tests together, the no observed effect concentration is 5 µg/mL.
Keywords
Silver Nanoparticles; Red Blood Cells; Platelets; Coagulation
Hrčak ID:
157636
URI
Publication date:
1.1.2014.
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