Original scientific paper

https://doi.org/10.20471/acc.2016.55.01.1

Immunohistochemical Differentiation of Triple Negative Breast Cancer

Miroslav Lesar ; Department of Oncology Surgery,University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Mladen Stanec ; Department of Oncology and Plastic Surgery, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Nikola Lesar ; Department of Anesthesiology,University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zaqgreb, Croatia
Danko Velimir Vrdoljak ; Department of Oncology Surgery, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Zvonimir Zore ; Department of Oncology Surgery,University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia,
Marija Banović ; student, School of Medicine, University of Zagreb, Zagreb, Croatia
Gordana Brozović ; Department of Anesthesiology, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia; Faculty of Medicine, University of Osijek, Osijek, Croatia

Abstract

Based on immunohistochemical staining for the basal markers cytokeratin 5/6 (CK 5/6), cytokeratin 14 (CK 14) and P-cadherin, triple negative tumors (TNT) are divided into two groups: 1) basal-like (BL) positive for one or all three markers; and 2) non basal-like (NBL)
negative for all three markers. Even though the different origin of the cells of these two types of tumors implies different biological properties, they had been treated as one entity until recently. This
paper analyzes TNT collected from 150 patients and distributed into two groups according to the results of immunohistochemical analysis, i.e. BL 116 (77.3%) and NBL 34 (22.67%). In this study, CK 5/6, CK 14 and P-cadherin were used as markers for identifying BL tumors. The immunohistochemical reaction was positive for CK 5/6 in 37%, for CK 14 in 50.86% and for P-cadherin in 68.34% of cases. The subclassification of triple negative breast cancer using the basal markers CK 5/6, CK 14 and P-cadherin has enabled identification of BL and NBL breast cancers in a proportion that is in line with the only accurate analysis of TNT gene expression. Using the mentioned combination of markers in daily practice is easy to perform and economically affordable.

Keywords

Carcinoma, ductal, breast; Triple negative breast neoplasms; Biomarkers, tumor; Basallike tumors; Non basal-like tumors; Adult

Hrčak ID:

161260

URI

https://hrcak.srce.hr/161260

Publication date:

1.3.2016.

Article data in other languages: croatian

Visits: 4.325 *