Original scientific paper

https://doi.org/10.21860/52;3_390

Impact of bone marrow angiogenesis, expression of osteopontin and vascular endothelial growth factor on myeloma patient response to the first line therapy

Emina Babarović ; Zavod za patologiju i patološku anatomiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Ita Hadžisejdić ; Zavod za patologiju i patološku anatomiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Sanja Štifter ; Zavod za patologiju i patološku anatomiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Ksenija Lučin ; Zavod za patologiju i patološku anatomiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Nives Jonjić ; Zavod za patologiju i patološku anatomiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka

Abstract

Objective: To investigate the effect of angiogenic parameters, expression of osteopontin (OPN) and vascular endothelial growth factor (VEGF) in the bone marrow in order to predict response to first-line therapy in patients with multiple myeloma (MM). Patients and Methods: The study included 68 patients with MM, treated at the Department of Hematology, Clinical Hospital Centre in Rijeka. For quantification of angiogenic parameters besides the classical method of determining microvessel density (MVD) we used a computer assisted image analysis which enabled us to calculate the number of blood vessels per mm2, the surface of blood vessels per mm2, an average diameter of blood vessels and total vascular surface (TVA) in the bone marrow biopsies stained with anti-CD34. To determine the expression of OPN and VEGF in plasma cells we used double staining immunohistochemistry method, CD138+OPN and CD138+VEGF. Results: We found that a higher angiogenic parameters, the MVD (P = 0.004) and the number of blood vessels per mm2 (P = 0.028), were significantly negative prognostic indicator of response to the first-line therapy in patients with MM. Conclusion: An objective method of the assessment of angiogenesis in the bone marrow samples could become a part of the routine histopathological analysis which could help to identify a subgroup of patients with possible aggressive course and poor response to the therapy.

Keywords

angiogenesis; immunohistochemistry; multiple myeloma; osteopontin; prognosis; vascular endothelial growth factor

Hrčak ID:

163943

URI

https://hrcak.srce.hr/163943

Publication date:

1.9.2016.

Article data in other languages: croatian

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