Original scientific paper
Fluorlmetric deterimination of methyl paraoxon
Zdenka Skurić
; Škola narodnog zdravlja »Andrija Štampar«, Medicinski fakultet Sveučilišta, Zagreb
Abstract
A fluorimetric method for the determination of methyl paraoxon is proposed. The method is based on the oxidation of indole with hydrogen peroxide, Some products of this reaction are fluorescent. The reaction is influenced by methyl paraoxon, the reaction rate being a function of the methyl paraoxon concentration in the solution. The sensitivity of the method is 1.1 micrograms methyl paraoxon in 50 cc of the final solution. Some organophosphorous compounds (paraioxon, parathion, methyl paraoxon, methyl parathion) yield p-nitrophenol as the product of their hydrolysis, It is experimentally shown that p-nitrophenol as a yellow compound decreases the fluorescence intensity of the fluorescent products of indole oxidation acting as an internal filter in the solution in which the organophosphorus compound is to be determined. Therefore it is suggested that, prior to the fluorimetric analysis of methyl paraoxon, the concentration of p-nitrophenol in the sample should be determined and the concentration of the organopbosphorous compound estimated from a calibration curve prepared with solutions of methyl paraoxon containing the same concentration of p-nitrophenol.
Keywords
Hrčak ID:
179401
URI
Publication date:
16.3.1965.
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