Original scientific paper

https://doi.org/10.24099/vet.arhiv.160414

Učinak odabranih makrolida na neka obilježja goveđih leukocita

Renata Urban-Chmiel ; Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
Andrzej Wernicki ; Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
Urszula Lisiecka ; Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences, Lublin, Poland
Andrzej Puchalski ; Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
Marta Dec ; Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
Diana Stęgierska ; Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
Łukasz Kurek ; Sub-Department of Internal Diseases of Farm Animals and Horses, University of Life Sciences, Lublin, Poland

Abstract

Cilj je ovog istraživanja bio procijeniti učinak tilozina, tilmikozina i roksitromicina na preživljavanje, test redukcije “nitro-tetrazol-modrog” (NBT), kemotaksiju, apoptozu i oksidacijski stres goveđih leukocita u uvjetima in vitro. Prilikom rutinskog veterinarskog pregleda, u epruvete s EDTA bila je uzeta krv iz jugularne vene goveda holštajnsko-frizijske pasmine u dobi od tjedan dana do 2,5 godine. Određen je postotak preživljavanja leukocita, koncentracija iona nitrata (NO), metabolička aktivnost (test redukcije “nitro-tetrazol-modrog”, NBT), kemotaksijska aktivnost i apoptoza. Rezultati su pokazali blagi negativni učinak spomenutih makrolida na preživljavanje leukocita i potvrdili sposobnost makrolida da potaknu njihovu apoptozu in vitro te naznačuju da svi pretraženi makrolidi imaju modulacijski učinak na funkciju leukocita goveda različite dobi. Najjači inhibicijski učinak na test redukcije NBT i kemotaksiju leukocita pokazao je roksitromicin, koji je istodobno imao najmanje negativan učinak na leukocite.

Keywords

apoptoza; goveđi leukociti; makrolidi; oksidacijski stres

Hrčak ID:

186219

URI

https://hrcak.srce.hr/186219

Publication date:

6.9.2017.

Article data in other languages: english

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