Original scientific paper
Determination of lead in blood
O.A. Weber
; Institut za higijenu rada Zagreb
K. Voloder
; institut za higijenu rada Zagreb
V.B. Vouk
; Institut za higijenu rada Zagreb
Abstract
A »mono-colour« dithizone method for determination of lead in blood is described. The main points in which the proposed method differs from the usual dithizone methods are the following:
(1) the extraction of lead with dithizone solution is performed at rather high pH values (10.5),
(2) the iron is removed after mincra lization of blood by means of a 2% aqueous cupferron solution.
The modification (I) increases the sensitivity of the method and at the same time dispenses with the necessity of washing lead-dithizonate solution to remove the excess dithizone before measuring the extinction. The cupferron extraction eliminates all the possible sources of error which are connected with the presence of iron. The statistical treatment of calibration curves showed that the proposed method is reliable, sensitive and precise. The standard error of a single determination is not more than ±7 µg for lead concentrations from 25 to 500 µg per 100 ml blood if the measurement of the optical density is performed with Beckman spectrophotometer at 520 mµ. If two parallel determinations arc made in each analysis the standard error may be reduced to ±5 µg.
Keywords
Hrčak ID:
187542
URI
Publication date:
24.9.1952.
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