Medicinski vjesnik, Vol. 38 No. (1-4), 2006.
Original scientific paper
The level of interleukin-1after intravesical klh instillation in patients with superficial transitional cell cancer of the urinary bladder
Carlos Winkler
Karl Friedrich Klippel
Abstract
Our study confirms the theory that instillation of KLH stimulates production of cytokines, resulting in their secretion in urine. Interleukin- 1 (IL-1) stimulates the immune cascade through a domino effect and is produced mainly by activated macrophages. The instillation program was started 5-7 days after TUR of primary superficial cell carcinoma. Twenty mg KLH in 20 ml of 0.9%
NaCl was instilled into the bladder each week for 6 consecutive weeks and then monthly for one year. When KLH is instilled into the bladder, IL-1alpha is secreted in the urine. A specific enzyme-linked immunosorbent assay (ELISA) was used for analysis. The ELISA for IL-1a was established in our laboratory and showed a detection limit of 5 pg/ml. This IL-1a ELISA deviation amounts to 3-7% within a series of measurements, and 5-15% from series to series. In the therapy group the IL-1a secretion ranged from 0 to 30,905 pg/24 h and in the control group from 0 (collection period) to 2,472 pg/4 h. IL 1a production increased significantly after KLH instillation in bladder cancer patients; however, the level varied considerably from patient to patient. Maximum production was achieved within a period of 4-8 h, decreasing within 24 h. There was a striking difference between the amount of IL-1a produced over the 24-hour period in the control group and that of the KLH group. 8 of 14 patients (57%) who responded to KLH therapy, had higher urine IL-1a levels after 6 weeks of KLH treatment than those who failed to respond within 12 months, but the levels were not of statistical significance. The secretion of IL-1a in urine is the biological response of the bladder to the antigen stimulus of KLH. No IL-2 was detected in the urine samples. It remains to be determined whether no IL-2 cytokine was present, or whether the amount was smaller than the minimal detection limit required for the ELISA.
Keywords
Keyhole Limpet Hemocyanine; Interleukin 1α; Interleukin 2; Transitional cell carcinoma of the urinary bladder
Hrčak ID:
191044
URI
Publication date:
1.12.2006.
Visits: 1.302 *