Veterinary Archives, Vol. 88 No. 1, 2018.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.160823a
An electrochemical immunosensor for detecting progesterone in milk from dairy cows
Ling Wu
; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China
Wei Yang
; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China
Cheng Xia
; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China
Chuang Xu
; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China
Hongyou Zhang
; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China
Abstract
In this study, an electrochemical immunosensor for milk progesterone produced by dairy cows was developed. Using the immunosensor, milk progesterone levels in healthy estrus dairy cows was found to range from 1 to 6 ng/mL 20 days after estrus. There were high levels of progesterone in the milk from cows with prolonged luteal phase and luteal cysts, which ranged from 15 to 28 and 19 to 29 ng/mL, respectively. Cows with inactive ovaries also showed low milk progesterone levels of 1-8 ng/mL, but they had lower plasma follicle-stimulating hormone (FSH) levels than the healthy estrous cycle dairy cows.
Keywords
dairy cows; electrochemical immunosensor; progesterone; reproductive status
Hrčak ID:
192709
URI
Publication date:
22.1.2018.
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