Original scientific paper

Qualitative and Quantitative Aspects of Using Eshtblausalz B to Visualise 4-Hydroxycoumarin and Some of its Biologically Active Derivatives on Thin-Layer Chromatographs

M. Trkovnik ; Laboratory of Organic Chemistry and Biochemistry, Technological Faculty, University of Banja Luka, 78000 Banja Luka, Bosnia and Hercegovina, Yugoslavia
M. Kuleš ; Laboratory of Organic Chemistry and Biochemistry, Technological Faculty, University of Banja Luka, 78000 Banja Luka, Bosnia and Hercegovina, Yugoslavia
I. Tabaković ; Laboratory of Organic Chemistry and Biochemistry, Technological Faculty, University of Banja Luka, 78000 Banja Luka, Bosnia and Hercegovina, Yugoslavia
B. Bobarević ; Laboratory of Organic Chemistry and Biochemistry, Technological Faculty, University of Banja Luka, 78000 Banja Luka, Bosnia and Hercegovina, Yugoslavia

Abstract

Qualitative and quantitative aspects of the analysis of 4-hy..:.
droxycoumarin and some of its biologically active derivatives
(dicumarol, TromexanR, warfatin, 3-(co-bromoacetyl)-4-hydroxycoumarin
and 3-(co-bromoacetyl)-4-hydroxy-7-bromocoumarin) were
studied. The coumarin derivatives can be sharply separated by
TLC on .silica gel HF254 using Eshtblausalz B for detection. Azo
compounds of coumarine derivatives show that a linear relationship
exists between spot-eluate absorbances and the amonts applied
from 10 to 30 μg. The new rapid and sensitive method was
obtained for qualitative and quantitative analysis of 4-hydroxy-
. coumarin derivatives.

Keywords

Hrčak ID:

194545

URI

https://hrcak.srce.hr/194545

Publication date:

15.11.1980.

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