Original scientific paper

Action of 1,6-Dimethyl-SB-(Bromoisonicotinoyl-Oxymethyl)-10a- Methoxyergoline (SermionR) on Some Molecular Processes in the Brain

B. Mildner ; Gustav-Emden-Zentrum der Bioiogischen Chemie, Abteitung fur Molekularbiologie, Universitat Frankfurt/Main, Federal Republic of Germany
A. Paul ; Gustav-Emden-Zentrum der Bioiogischen Chemie, Abteitung fur Molekularbiologie, Universitat Frankfurt/Main, Federal Republic of Germany
P. Chandra ; Gustav-Emden-Zentrum der Bioiogischen Chemie, Abteitung fur Molekularbiologie, Universitat Frankfurt/Main, Federal Republic of Germany

Abstract

The influence of SermionR on cellular protein synthesis in the
mouse brain was studied in vivo, after an intraperitoneal administration
of 50 microgram doses. The brain samples analyzed at different
intervals after drug treatment showed a short-term increase of
3H-Leucine incorporation into proteins, followed by a. gradual
decline of uptake. The latter reached control levels about 18 h
after drug administration. Under similar experimental treatments
Sermion had no effect on the synthesis of RNA catalyzed by Mg++-
ions; however, a slight stimulation of the Mn++- activated RNA
synthesis was obserwed.
Mice receiving i. p. injections of 50 micrograms of Sermion
showed an increased brain rnethyltransferase activity; the maximum
stimulation was observed one hour after drug treatment. In another
set of experiments the capacity of mouse brain preparations to
catalyze the methyl group incorporation into the exogenously added
transfer RNA (tRNA) was found to decrease with increasing age of
the animals; the addition of 10-4 M Sermion to the incubation medium
enhanced endogenous rnethylation, especially in the older
animals. On the other hand the capacity to methylate the exogenously
added heterologous tRNA was not effected by Sermion, irrespective
of the age of the animals.
In an attempt to understand the physiological relevance of the
biochemical effects of Sermion, we have carried out some studies
on the effect of this drug on conditioned learning in rats. The preliminary
data indicate that the drug is able to enhance the learning
quotients, however, more experiments are in progress to reach a
definitive conclusion.

Keywords

Hrčak ID:

196354

URI

https://hrcak.srce.hr/196354

Publication date:

20.7.1977.

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