Conference paper

Mixed Solvents as Tools to Study the Structural Aspects of Cytochrome P450 in Solution at Sub-Zero Temperatures

R. Lange ; Service de Biospectroscopie, Institut de Biologie Physico-chimique, 13 Rue Pierre et Marie Curie, 75005 Paris, France
P. Debey ; Service de Biospectroscopie, Institut de Biologie Physico-chimique, 13 Rue Pierre et Marie Curie, 75005 Paris, France
P. Douzou ; Service de Biospectroscopie, Institut de Biologie Physico-chimique, 13 Rue Pierre et Marie Curie, 75005 Paris, France

Abstract

Cytochromes P450 from various sources are known to have
mixtures of different heme-iron electronic configurations, partly
in thermodynamic equilibrium, reflecting different configurations of
the protein.
Organic solvents (such as ethylene glycol or glycerol) as well
as temperature changes have a marked but reversible effect on the
thermodynamic parameters of these equilibria in the fluid state.
The solvent and temperature perturbation of the conformational
or spin state equilibrium have been investigated on various redox
states of cytochrome P450 in order to gain some insight · into the
structure of the heme pocket.

Keywords

Hrčak ID:

196367

URI

https://hrcak.srce.hr/196367

Publication date:

30.3.1977.

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