Conference paper

Treatment of Rat Liver Microsomes with Phospholipase C: Effect on Phospholipids and on Cytochromes P450 and b5

J. W. DePierre ; Arrheniuslaboratoriet, Avdelningen fur biokemi, Stockholms Universitet, Fack, S-1005 Stockholm, Sweden
L. Ernster ; Arrheniuslaboratoriet, Avdelningen fur biokemi, Stockholms Universitet, Fack, S-1005 Stockholm, Sweden

Abstract

Treatment of rat liver microsomes with phospholipase C (CL
weLchii) revealed the following:
1. The polar headgroups of 70°/o of the phospholipids can be
removed by treatment of microsomes with phospholipase C. When
phospholipids that have been extracted from microsomes are treated
with phospholipase C, 900/o can be hydrolyzed, suggesting that
certain phospholipids are protected from the enzyme in situ.
2. Neither the native conformations of cytochromes P450 and
b5 nor their binding to the microsomal membrane are directly
affected by phospholipase C treatment.
3. The diglycerides resulting from the action of phospholipase
C can be hydrolyzed by an enzyme in the microsomal membrane
to yield free fatty acids which partially denature cytochrome P450.
4. The pattern of this partial denaturation is a further indication
of the existence of a number of cytochrome P450 species in
the microsomal membrane.

Keywords

Hrčak ID:

196375

URI

https://hrcak.srce.hr/196375

Publication date:

30.3.1977.

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