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Croatica Chemica Acta, Vol. 46 No. 2, 1974.

Original scientific paper

Some Properties of Thymus Cathepsin D

I. Kregar ; Department of Biochemistry, J . Stefan I nstitute, University of Ljubljana, 61000 Ljubljana, Yugoslavia
V. Turk ; Department of Biochemistry, J . Stefan I nstitute, University of Ljubljana, 61000 Ljubljana, Yugoslavia
F. Gubenšek ; Department of Biochemistry, J . Stefan I nstitute, University of Ljubljana, 61000 Ljubljana, Yugoslavia
R. W. Smith ; Department of Biochemistry, J . Stefan I nstitute, University of Ljubljana, 61000 Ljubljana, Yugoslavia

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Abstract

Cathepsin D has been purified from calf thymus using
ammonium sulphate precipitation and gel chromatography on
Se.phadex G-100. Preparative electrophoresis in polyacrylamide
gel, used as the final step in the purification procedure, yielded
four active forms of cathepsin D that dissociated further .into
several polypepUde bands in the presence of sodium dodecyl
sulphate. All four forms were stable over a range of pH from
4-11. They were completely inhibited by pepstatin whereas other
metal ions had no appreciable effect upon their activity.

Keywords

Hrčak ID:

196827

URI

https://hrcak.srce.hr/196827

Publication date:

1.11.1974.

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