Professional paper
https://doi.org/10.21860/medflum2018_203560
Genotyping of toxigenic strains of Clostridium difficile
Tanja Petrović
; Zavod za mikrobiologiju i molekularnu dijagnostiku, Sveučilišna klinička bolnica Mostar, Bosna i Hercegovina
Sanja Jakovac
; Zavod za mikrobiologiju i molekularnu dijagnostiku, Sveučilišna klinička bolnica Mostar, Bosna i Hercegovina
Abstract
Aim: The aim of this paper was the molecular analysis of toxigenic strains of C. difficile to determine the distribution of different genotypes.
Subjects and Methods: The study was performed on stools samples from patients hospitalized in the University Clinical Hospital Mostar. Production of toxins A and B was detected by ELFA technique in all clinical samples. Isolated C. difficile DNA was amplified by PCR reaction. Hybridization method was used for determinatin of: C. difficile specific gene (tpi), genes resposible for production of toxins A and B (tcdA, tcdB) and binary toxins (cdtA, cdtB), deletions in tcdC gene at positions 18 bp, 39 bp and 117 bp, and two different mutations in the gyrA gene.
Results: In all of the strains C. difficile, the toxin A and B genes (tcdA, tcdB) were present. Presence of the mutation in the gene for moxifloxacin (gyrA) resistance was demonstrated in 96.66% C. difficile strains. The same strains have also been shown to produce binary toxins, confirming that these strains belong to the hypervirulent ribotype 027.
Conclusions: This study has shown that in the University Clinical Hospital Mostar among toxigenic strains of C. difficile the predominant genotype was moxifloxacin-resistant, hypervirulent ribotype 027.
Keywords
Clostridium difficile; genotype; hypervirulent ribotype
Hrčak ID:
203560
URI
Publication date:
1.9.2018.
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