Original scientific paper
https://doi.org/10.5937/sejodr5-19646
Evaluating the sterility of orthodontic materials as received from the manufacturer and that exposed to clinic environment - in vitro study
SS. Musaddique
K. Ajit
H. Ashwith
M. Nasim
AK. Faraaz Mohamed
Abstract
Introduction: Sterilization in orthodontics has been discussed and stressed over times in the dental literature. Sterility of orthodontic
materials should be of prime interest to the orthodontist as a health care professional and he should take appropriate measures to
prevent the risk of cross infection.
Aim: To evaluate the sterility of orthodontic materials as received from the manufacturer and that exposed to clinic environment for 6 months.
Materials and methods: The sterility of orthodontic materials "as received" from the manufacturer and "clinically exposed" (unused materials
stored in the clinic environment for a period of 6 months) was microbiologically evaluated (universal & conventional PCR).The materials
which were included in this study were the sealed and unsealed orthodontic archwires, molar bands, elastomeric module, stainless steel
bracket, coil spring and tungsten carbide burs of various brands (American Orthodontics, 3M Unitek, Ormco, Orthosystems, G&H,
Modern Orthodontics, SS white etc).
Results: After universal and conventional PCR, the detection of micro-organisms in all materials was significantly higher in the clinically
exposed samples compared to as received samples from manufacturers (p<0.001). The detection of micro-organisms in all orthodontic
wires is significantly higher in the unsealed samples compared to sealed samples (p<0.001). In universal PCR, the detection of microorganisms
in all materials (Coil spring, E-chain, Elastomeric, Molar brands, Tungsten carbide) except for SS brackets is significantly higher
in both as received and clinically exposed samples.
Conclusion: After universal and conventional PCR, we conclude that bacteria were present on almost all orthodontic materials "as received
from the manufacturers" and that exposed to a clinical environment. Therefore all materials should be sterilized before use in patients.
Keywords
orthodontic materials; sterility
Hrčak ID:
215838
URI
Publication date:
17.10.2018.
Visits: 1.178 *