Veterinarska stanica, Vol. 48 No. 5, 2017.
Review article
Methods for isolation and identification of bacteria of the genus Campylobacter - genotyping methods (Part II)
Marina Mikulić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Andrea Humski
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Bela Njari
; Veterinarski Fakultet Sveučilišta u Zagrebu, Hrvatska
Dora Stojević
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Luka Jurinović
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Silvio Spičić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Sanja Duvnjak
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Željko Cvetnić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Abstract
Distinction of bacterial strains below the species or subspecies level is made possible by diverse genotyping methods. Many public health laboratories use pulsed field gel electrophoresis (PFGE), which has been constantly improving since the 1980s. Other methods used include restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) method and ribotyping. The process of determining the order of nucleotides within DNA is called sequencing. Newly evolved methods based on multilocus sequence typing are MLST, rMLST
and cgMLST. These examine the relationships between bacteria of the genue Campylobacter, especially C. jejuni and C. coli. They are useful in investigating possible sources and reservoirs of disease, their connection and the molecular epidemiology of diseases. A new revolutionary method in microbiology is whole genome sequencing (WGS), and it is believed that this method will gradually replace other genotyping methods.
Keywords
Campylobacter spp.; strain differentiation; sequence typing; PFGE
Hrčak ID:
222644
URI
Publication date:
15.10.2017.
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