Original scientific paper
https://doi.org/10.2478/aiht-2019-70-3342
Effects of butylparaben on antioxidant enzyme activities and histopathological changes in rat tissues
Duygu Aydemir
; School of Medicine, Department of Medical Biochemistry, Koc University, Istanbul, Turkey 2 Koç University Research Center for Translational Medicine, Istanbul, Turkey
Burcu Öztaşcı
; Department of Biology, Science Faculty, Hacettepe University, Ankara, Turkey
Nurhayat Barlas
; Department of Biology, Science Faculty, Hacettepe University, Ankara, Turkey
Nuriye Nuray Ulusu
; School of Medicine, Department of Medical Biochemistry, Koc University, Istanbul, Turkey 2 Koç University Research Center for Translational Medicine, Istanbul, Turkey
Abstract
Butyl p-hydroxybenzoic acid, also known as butylparaben (BP), is one of the most common parabens absorbed by the skin and gastrointestinal tract and metabolised in the liver and kidney. Recent in vivo and in vitro studies have raised concern that BP causes reproductive, development, and teratogenic toxicity. However, BP-induced oxidative stress and its relation to tissue damage has not been widely investigated before. Therefore, we aimed to investigate the effects of butyl 4-hydroxybenzoate on enzyme activities related to the pentose phosphate pathway and on glutathione-dependent enzymes such as glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6-PGD), glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) in kidney, liver, brain, and testis tissues. Male rats were randomly divided into four groups to orally receive corn oil (control) or 200, 400, or 800 mg/kg/ day of BP for 14 days. Then we measured G6PD, GR, GST, 6-PGD, and GPx enzyme activities in these tissues and studied histopathological changes. BP treatment caused imbalance in antioxidant enzyme activities and tissue damage in the liver, kidney, brain, and testis. These findings are the first to show the degenerative role of BP on the cellular level. The observed impairment of equivalent homeostasis and antioxidant defence points to oxidative stress as a mechanism behind tissue damage caused by BP.
Keywords
endocrine disrupting chemicals, glucose 6-phosphate dehydrogenase; 6-phosphogluconate dehydrogenase; glutathione reductase; glutathione peroxidase; glutathione-S-transferase
Hrčak ID:
229440
URI
Publication date:
6.12.2019.
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