Original scientific paper

https://doi.org/10.24099/vet.arhiv.0502

In vitro protoscolicidal efficacy appraisal of methanolic herbal extracts against hydatid cysts

Aman D. Moudgil orcid.org/0000-0002-1130-2376 ; Department of Veterinary Parasitology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur (H.P.), India
Pallavi Moudgil ; Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar (Haryana), India
Dinesh Sharma ; Department of Veterinary Pharmacology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur (H.P.), India
Prashant S. Daundkar ; Department of Veterinary Pharmacology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur (H.P.), India
R. K. Agnihotri ; Department of Veterinary Parasitology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur (H.P.), India

Abstract

The present study aimed at an evaluation of the protoscolicidal potential of methanolic extracts of three herbs; Ferula asafoetida (dried latex), Trachyspermum ammi (fruits) and Hippophae salicifolia (leaves) at three different concentrations (10, 20 and 30 mg/mL) for 20, 40 and 60 minute treatment intervals, with respect to standard allopathic drug albendazole. The in-vitro viability of the protoscolices was assessed by the Eosin exclusion method. The slope of mortality and lethal concentration for 50% (LC50) was determined from the regression graphs of the probit mortality of protoscolices plotted against log values of increasing concentrations of herbal extracts for 60 minute treatment intervals. The percentage of mortality caused by various extracts at concentrations ranging from 10-30 mg/mL varied from 29.54-97.76% with maximum mortality of 97.76, 97.30 and 81.91% recorded with methanolic extracts of F. asafoetida, T. ammi and H. salicifolia, respectively. Among all the extracts, the highest protoscolicidal activity was exhibited by F. asafoetida at 20 and 30 mg/mL concentrations in the 60 (97.16%) and 40/60 minutes (97.20% and 97.76%) treatments, respectively, and it showed a minimum LC50 value of 0.204, followed by T. ammi at 0.933. As compared to the standard allopathic drug, albendazole, the methanolic extracts of F. asafoetida and T. ammi exhibited better or comparable protoscolicidal activities.

Keywords

cystic echinococcosis; herbs; lethal concentration; protoscolicidal activity

Hrčak ID:

238616

URI

https://hrcak.srce.hr/238616

Publication date:

25.5.2020.

Article data in other languages: croatian

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