Original scientific paper

A Contribution to the Biochemical Characterization of Allergens in Pollen Extracts of Secale Cereale

N. Muić ; Department of Applied Biochemistry, »Andrija Stampar« School of Public Health, Faculty of Medicine, University of Zagreb and Institute for the Production of Sera and Vaccines, Zagreb, Croatia, Yugoslavia
N. Kohler-Kubelka ; Department of Applied Biochemistry, »Andrija Stampar« School of Public Health, Faculty of Medicine, University of Zagreb and Institute for the Production of Sera and Vaccines, Zagreb, Croatia, Yugoslavia

Abstract

Buffe:r; extracts of pollen from Secale cereale as used ii+ therapy of pollinosis were prepa~edl. All the extracts were found active upon intradermal test in pollen sensitive subjects. Electro;phoretic and chromatographic analysis revealed that the extracts contain seven ninhydrin reacting components. Two amino acids, proline and [ysine, and five others (very probably polypeptides) were found to be components of the extract. Two major peptide components (C2 and D) were isolated and amino acids composition of acid hydrolyzates determined. Component C2 contained Asp., Glu., Ser., Gly., Thr., Lys., Tyr., (Cys.?), and D component Asp., Glu., Ser., (Cys.?), Gly., Thr., Tyr., V,al., Pro. I:n the original pollen extract's hydrolyzate His., Phen., Leu., not certainly Tryp,, and Isoleu. were found in addition to the amino acids cited above. Of carbohydrates glucose, galactose and fructose were detected.

Keywords

Hrčak ID:

244854

URI

https://hrcak.srce.hr/244854

Publication date:

8.4.1960.

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