Original scientific paper

https://doi.org/10.2478/acph-2021-0036

Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma

SERDAR KARAKURT orcid.org/0000-0002-4449-6103 ; Selcuk University, Faculty of Science, Department of Biochemistry, Konya, Turkey
SINAN KANDIR orcid.org/0000-0002-8404-7994 ; Cukurova University, Ceyhan Faculty of Veterinary Medicine, Department of Physiology, Adana, Turkey
ÇİĞDEM GÖKÇEK-SARAÇ ; Faculty of Engineering, Department of Biomedical Engineering, Akdeniz University, Antalya, Turkey

Abstract

The present study's objective is to clarify the molecular mechanisms of tannic acid effects on the viability of human colorectal carcinoma (CRC). Tannic acid is stable for up to 48 h and is localized in both cytoplasm and nucleus. It dose-dependently inhibited the viability of CRC cell lines; SW-620 and HT-29 with IC50 values of 7.2 ± 0.8 and 37.6 ± 1.4 µmol L–1. Besides, metastatic, invasive, and colony formation properties of CRC cells were significantly inhibited following the tannic acid treatment (p < 0.001). Tannic acid has been found to modulate enzyme, protein, and gene expressions of NQO1 in different levels and the upregulation of protein/gene expressions of p53 (p < 0.001), which leads the cells to trigger apoptosis. In conclusion, the present in vitro study may supply a significant background for in vivo studies in which the molecular mechanisms of antioxidant and chemopreventive activities of tannic acid will completely clarify.

Keywords

tannic acid; colorectal carcinoma; wound healing; cell viability; NQO1; p53

Hrčak ID:

245684

URI

https://hrcak.srce.hr/245684

Publication date:

31.12.2021.

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