Acta clinica Croatica, Vol. 59. No. 3., 2020.
Review article
https://doi.org/10.20471/acc.2020.59.03.12
The Role of New Technologies in Defining Salivary Protein Composition Following Placement of Fixed Orthodontic Appliances – Breakthrough in the Development of Novel Diagnostic and Therapeutic Procedures
Vojka Zgombić Popović
orcid.org/0000-0002-9355-3290
; Orthonova Dental Polyclinic, Zagreb, Croatia
Lovorka Grgurević
orcid.org/0000-0002-9814-3178
; Drago Perović Department of Anatomy, School of Medicine, University of Zagreb, Zagreb, Croatia; Center for Translational and Clinical Research, Department of Proteomics, School of Medicine, University of Zagreb, Zagreb, Croatia
Vladimir Trkulja
; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Ruđer Novak
orcid.org/0000-0002-5920-4169
; Center for Translational and Clinical Research, Department of Proteomics, School of Medicine, University of Zagreb, Zagreb, Croatia
Dubravka Negovetić-Vranić
; Department of Pediatric and Preventive Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Abstract
Human saliva is rich in proteins of variable functions (e.g., enzymes, immunoglobulins, cytokines) and origin (blood plasma, salivary glands, or oral microflora). Circadian dynamics, volume and composition (electrolytes, pH, protein, etc.) of secreted saliva vary with local and systemic physiological and pathophysiological conditions. Therefore, the composition of saliva, protein in particular, has been intensively investigated to identify the potential markers and/or mechanisms of systemic and local diseases. Proteomic techniques used for the analysis of biological fluids have enabled great advances in salivary protein stabilization (as the main precondition for their analysis) and detection of those found in saliva in very low concentrations, including small proteins and peptides. This review brings the main characteristics of current proteomic techniques such as liquid chromatography-mass spectrometry, two-dimensional electrophoresis-mass spectrometry, and surfaceenhanced laser desorption ionization/time of flight/mass spectrometry. These techniques enable simultaneous identification of hundreds and thousands of protein molecules, as well as identifying those of a potential biological value in particular states. This literature review is focused on the state-of-the-art and possibilities offered by proteomic techniques in analyzing the effects of orthodontic appliances on salivary protein composition and searching for potential markers of therapeutic success/failure or for the molecules by which therapeutic
effects are achieved.
Keywords
Saliva; Proteomics; Orthodontic appliance; Salivary protein
Hrčak ID:
247461
URI
Publication date:
1.9.2020.
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