Original scientific paper

Head and Neck Tumor Cells Exhibit Altered Proliferation upon Overexpression of nm23 Genes

Maja Herak Bosnar orcid.org/0000-0002-8448-4713 ; Ruđer Bošković Institute, Zagreb, Croatia
Klara Dubravčić ; Clinical Hospital »Rebro«, Zagreb, Croatia
Ružica Bago ; Ruđer Bošković Institute, Zagreb, Croatia
Jasminka Pavelić ; Ruđer Bošković Institute, Zagreb, Croatia

Abstract

nm23 was identified as a metastasis suppressor gene but is also appointed to a number of other biological functions. The goal of this study was to reveal the influence of ectopic expression of nm23-H1 and nm23-H2 on proliferation properties of head and neck tumor cells. The proliferation rate of transfected cells was evaluated using EGFP reporter system and flow cytometry. HEp-2 and CAL 33 cells transiently transfected with nm23 cDNA containing constructs exhibited enhanced proliferation. CAL 27 cells constitutively expressing GFP-Nm23-H2 protein, exhibited intense proliferation the first day after seeding, while the GFP-Nm23-H1 expressing clone started to proliferate after one-day lag period. The results on transiently transfected HEp-2 and CAL 33 cells generally confirmed previous findings connecting nm23 expression with altered proliferation of head and neck tumors. We speculate that the effects observed on stably transfected CAL 27 clones are due to their different attachment properties.

Keywords

nm23; head and neck tumor cell lines; flow cytometry; green fluorescent protein

Hrčak ID:

23446

URI

https://hrcak.srce.hr/23446

Publication date:

15.4.2008.

Visits: 1.971 *