Veterinary Archives, Vol. 91 No. 1, 2021.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.0829
Carbapenem resistant Escherichia coli and Pseudomonas aeruginosa in captive blackbucks (Antilope cervicapra) and leopards (Panthera pardus) from India
Obli R. Vinodh Kumar
; Division of Epidemiology, ICAR- Indian Veterinary Research Institute, Bareilly, India
Bhoj R. Singh
; Division of Epidemiology, ICAR- Indian Veterinary Research Institute, Bareilly, India
Mathesh Karikalan
; Centre for Wildlife, ICAR- Indian Veterinary Research Institute, Bareilly, India
Shikha Tamta
; Division of Epidemiology, ICAR- Indian Veterinary Research Institute, Bareilly, India
Jai K. Jadia
; Veterinary Officer, Nandanvan Zoo, Raipur, Chhattisgarh, India
Dharmendra K. Sinha
; Division of Epidemiology, ICAR- Indian Veterinary Research Institute, Bareilly, India
Karunanidhi Mahendran
; Division of Medicine, ICAR- Indian Veterinary Research Institute, Bareilly, India
Ramkumar N. Rupner
; Division of Epidemiology, ICAR- Indian Veterinary Research Institute, Bareilly, India
Ravichandran Karthikeyan
; Division of Epidemiology, ICAR- Indian Veterinary Research Institute, Bareilly, India
Anil K. Sharma
; Centre for Wildlife, ICAR- Indian Veterinary Research Institute, Bareilly, India
Abstract
The study aimed to investigate the occurrence of carbapenem resistant E. coli and P. aeruginosa in apparently healthy, captive blackbucks and leopards of India. Faecal samples of blackbucks (n = 7) and leopards (n = 7) were processed to isolate carbapenem resistant E. coli (CRE) and P. aeruginosa (CRP). Forty (leopards n = 26; blackbuck n = 14) E. coli and two P. aeruginosa (blackbuck n = 2) samples were isolated from the faecal samples (n = 14). Eleven carbapenem resistant isolates were recovered, of which 10 were CRE and one was CRP. The minimum inhibitory concentration (MIC) was determined for meropenem for carbapenem resistant isolates and was between 8 and 64 μg/mL. All the CRE and CRP were phenotypically multidrug resistant, and six CRE were extended-spectrum beta- lactamases (ESBL) producers. On genotypic screening, seven CRE and one CRP were positive for the blaNDM carbapenemase gene. Efflux pump-mediated carbapenem resistance was noticed in four CRE isolates (36.4%, 4/11). Of the six ESBL producing CRE, four isolates carried blaCTX-M-1 genes. The CRE isolates also harbored blaTEM-1, blaAmpC, qnrA, qnrB, qnrS, tetA, tetB and sul1 resistance genes. On Shiga toxin virulence screening, Stx1, Stx2 genes were detected in two and one isolates, respectively. Plasmid typing of CRE revealed that the blaNDM genes were carried on an Incl1 plasmid. The plasmid multilocus sequence typing (pMLST) of the isolates showed the Sequence Type (ST) 297. The occurrence of carbapenem resistance bacteria in captive wildlife should be a major public health priority.
Keywords
ESBL; E. coli; Shiga toxin; wildlife; Carbapenem-resistance; NDM
Hrčak ID:
255226
URI
Publication date:
6.4.2021.
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