Veterinarska stanica, Vol. 53 No. 2, 2022.
Original scientific paper
https://doi.org/10.46419/vs.53.2.8
Difference of seminal plasma and sperm proteins in good and poor freezability boar ejaculates
Janyaporn Rungruangsak
; Semen Quality Control and Research Section, Bureau of Biotechnology in Livestock Production, Department of Livestock Development, Muang, Pathum Thani, 12000 Thailand
Junpen Suwimonteerabutr
; Swine Reproduction Research Unit, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
Kakanang Buranaamnuay
; Institute of Molecular Biosciences, Mahidol University 25/25 Phuttamonthon 4 Rd., Salaya, Nakhon Pathom 73170, Thailand
Sariya Asawakarn
; Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand
Naphat Chantavisoote
orcid.org/0000-0003-3946-1798
; Chulalongkorn University Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand
Trairak Pisitkun
; Chulalongkorn University Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand
Kamon Chaweewan
; Swine Research and Development Centre, Department of Livestock Development, Nakhon Ratchasima, Thailand
Padet Tummaruk
orcid.org/0000-0001-7000-4371
; Swine Reproduction Research Unit, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
Abstract
The present study was performed to compare the expression of sperm proteins, i.e. triosephosphate isomerase (TPI) and acrosin binding protein (ACRBP) and seminal plasma proteins, i.e. glutathione peroxidase 5 (GPX5) and fibronectin 1 (FN1), in boar semen with good, moderate and poor freezability. The study was conducted by determining the protein contents in 32 sperm samples and 38 seminal plasma samples of semen. The ejaculated semen was divided into two portions: the first portion was centrifuged to separate the pellet of sperm from the seminal plasma and the second portion was cryopreserved. After thawing, the ejaculates were classified into three groups according to their post-thawed sperm motility: good (60.2 ± 1.7%), moderate (29.3 ± 2.0%) and poor (16.6 ± 2.2%) freezabilities. The expressions of GPX5 and FN1 in seminal plasma and TPI and ACRBP in sperm were determined using Western blot analysis. It was found that, for sperm proteins, the level of TPI was negatively correlated with the post-thawed total sperm motility (r = -0.38, P = 0.029). For seminal plasma proteins, the level of FN1 in the seminal plasma was positively correlated with the post-thawed total sperm motility (r = 0.37, P = 0.021) and progressive motility (r = 0.39, P = 0.016). The expression of GPX5 was not correlated with any of the frozen–thawed sperm qualities (P > 0.05). In conclusions, boar semen containing a high level of FN1 in seminal plasma has better freezability. Frozen–thawed sperm motility was positively correlated with the level of FN1 in boar seminal plasma and negatively correlated with TPI in boar spermatozoa.
Keywords
boar; cryopreservation; freezability; protein; sperm
Hrčak ID:
261485
URI
Publication date:
24.6.2021.
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