Short communication, Note

https://doi.org/10.2478/acph-2022-0023

Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica

CHEN GAO ; Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, 014040; Department of Pharmacy, The Second Affiliated Hospital of Baotou Medical College, China, 014030
HONG CHANG ; Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, 014040
HONG-BING ZHOU ; Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, 014040
QING LIU ; Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, 014040
YING-CHUN BAI ; Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, 014040
QUAN-LI LIU ; Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, 014040; Institute of Bioactive Substance and Function of Mongolian Medicine and Chinese Materia Medica, Baotou Medical College, Baotou, Inner Mongolia, China, 014040
WAN-FU BAI ; Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, 014040
SONG-LI SHI ; Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, 014040; Institute of Bioactive Substance and Function of Mongolian Medicine and Chinese Materia Medica, Baotou Medical College, Baotou, Inner Mongolia, China, 014040

Abstract

To reveal the mechanism of anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica, renal fibrosis was induced with unilateral ureteral obstruction (UUO) and then treated with an n-butanol extract (BUT) from Amygdalus mongolica (Rosaceae). Sixty male Sprague-Dawley rats were randomly divided into the sham-operated, renal fibrosis (RF) model, benazepril hydrochloride-treated model (1.5 mg kg–1) and BUT-treated (1.75, 1.5 and 1.25 g kg–1) groups and the respective drugs were administered intragastrically for 21 days. Related biochemical indices in rat serum were determined and histopathological morphology observed. Serum metabolomics was assessed with HPLC-Q-TOF-MS. The BUT reduced levels of blood urea nitrogen, serum creatinine and albumin and lowered the content of malondialdehyde and hydroxyproline in tissues. The activity of superoxide dismutase in tissues was increased and an improvement in the severity of RF was observed. Sixteen possible biomarkers were identified by metabolomic analysis and six key metabolic pathways, including the TCA cycle and tyrosine metabolism, were analyzed. After treatment with the extract, 8, 12 and 9 possible biomarkers could be detected in the high-, medium- and low-dose groups, respectively. Key biomarkers of RF, identified using metabolomics, were most affected by the medium dose. A. mongolica BUT extract displays a protective effect on RF in rats and should be investigated as a candidate drug for the treatment of the disease.

Keywords

Amygdalus mongolica; renal fibrosis; protective effect; metabolomics; mechanism

Hrčak ID:

265130

URI

https://hrcak.srce.hr/265130

Publication date:

30.9.2022.

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