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Psychiatria Danubina, Vol. 26 No. suppl 3, 2014.

Original scientific paper

MELITOCOCCOSIS IN THE REPUBLIC OF CROATIA

Željko Cvetnić ; Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Zagreb, Croatia
Silvio Špičić ; Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Zagreb, Croatia
Tomislav Kiš ; Ministry of Agriculture - Directorate for Veterinary Medicine and Food Safety, Zagreb, Croatia
Maja Zdelar-Tuk ; Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Zagreb, Croatia
Sanja Duvnjak ; Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Zagreb, Croatia
Ivana Račić ; Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Zagreb, Croatia
Miroslav Benić ; Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Zagreb, Croatia
Boris Habrun ; Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Zagreb, Croatia
Irena Reil ; Croatian Veterinary Institute Zagreb (Department of Bacteriology and Parasitology), Zagreb, Croatia
Zvonimir Šostar ; Andrija Stampar Teaching Institute of Public Health, Zagreb, Croatia

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Abstract

Background: Melitococcosis is one of the most widespread zoonoses worldwide. In the period from 2009 to 2013, comprehensive
melitococcosis testing was conducted in the Republic of Croatia.
Methods and results: During the testing, the Rose Bengal test was applied to 344019 blood samples of sheep and goats, and
positive reactions were confirmed in 1143 (0.3%) of samples. The complement fixation test (confirmatory test) was conducted on
43428 samples, with positive reactions confirmed in 768 (1.8%) of samples. The organs and tissues of 336 sheep and goats were
inspected bacteriologically, and Brucella sp. was isolated in 15 (4.5%) of samples. Positive serological and bacteriological reactions
were confirmed in the Karlovac, Lika-Senj and Split-Dalmatia Counties. Bacteriological and molecular techniques (Bru-up/Bru-low
and Bruce-Ladder) in isolates proved the presence of Brucella melitensis biovar 3.
Conclusion: On the basis of this study, it can be concluded that Croatia has a favourable situation concerning the infection of
ruminants with B. melitensis, and that ongoing controls of the disease are necessary.

Keywords

melitococcosis; Brucella melitensis; ruminants; serological and bacteriological tests; Republic of Croatia

Hrčak ID:

265293

URI

https://hrcak.srce.hr/265293

Publication date:

23.12.2014.

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