Original scientific paper

Rapid detection of Mycobacterium bovis in clinical samples using thp-1 cells

Manoharan Parthiban ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Rengarajan Jaiganesh ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Wilson Santhosh Kumar Aruni ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Sundaram Meignanalakshmi ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
Pachikani Ramadass ; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India

Abstract

The rapid isolation of M. bovis was attempted in THP-1 cells (Macrophage cell line). THP-1 cells infected with M. bovis showed characteristic cytopathic effect 48 hr after infection. The bacteria had grown very well in the first passage itself, without any blind passages in cell culture. The presence of bacteria in the cell culture fluid was confirmed by Ziehl-Neelsen staining and nested PCR amplification of insertion sequences.

Keywords

M. bovis; THP-1 cells; nested PCR; Ziehl-Neelsen stain; decontamination

Hrčak ID:

24847

URI

https://hrcak.srce.hr/24847

Publication date:

20.6.2007.

Article data in other languages: croatian

Visits: 1.915 *