Veterinary Archives, Vol. 91 No. 5, 2021.
Original scientific paper
https://doi.org/10.24099/vet.arhiv.1038
Association of genetic variants in STAT5B gene with milk performance and mastitis related traits in dairy cattle
Nawab Ali
; College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Pakistan; Department of Zoology, Abdul Wali Khan University Mardan, Pakistan; College of Animal Sciences and Technology, China Agriculture University Beijing, China
Sadaf Niaz
; Department of Zoology, Abdul Wali Khan University Mardan, Pakistan
Irfan Khattak
; College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Pakistan
Naimat U. Khan
; College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Pakistan
Hanpeng Luo
; College of Animal Sciences and Technology, China Agriculture University Beijing, China
Hazir Muhammad
; Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
Tariq Ali
; Center for Microbiology and Biotechnology, Veterinary Research Institute, Peshawar, Pakistan
Ying Yu
; College of Animal Sciences and Technology, China Agriculture University Beijing, China
Yachun Wang
; College of Animal Sciences and Technology, China Agriculture University Beijing, China
Tahir Usman
; College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Pakistan
Abstract
Udder performance and health are important traits in dairy cattle worldwide. The present study aimed to explore the association of single nucleotide polymorphisms (SNPs) in the STAT5B gene in dairy cattle with milk performance and mastitis related traits. The study included 201 cows of three pure breeds (i.e. Holstein Friesian, Jersey and Achai) and two crossbred cattle at four established dairy farms in Khyber Pakhtunkhwa, Pakistan. The milk samples were analysed for somatic cell count (SCC) and milk composition (i.e. fat , protein and lactose percentages). The generalized linear model was deployed for association analysis using SAS. The pool DNA sequencing showed four (three synonymous and a 3/ UTR) SNPs in STAT5B. These SNPs were further validated in all DNA samples using SNaPshot assay. The breed-wise analysis showed that most of the SNPs were consistent with Hardy-Weinberg equilibrium (P>0.05). The association analysis revealed a significantly higher protein percentage in TT genotype and lower SCC in CC genotypes of SNP 1 (exon 2, C>T), whereas in SNP 2 (exon 16, T>C) the TT genotypes revealed significantly lower SCC and SCS compared with other genotypes (P<0.05). SNP 4 (3/ UTR, C>T) showed significantly lower SCC and frequency of clinical mastitis in the heterozygous (CT) genotype compared to the homozygous genotypes. The in silico predictions revealed changes in the RNA secondary structure for SNP 2 and SNP 3. The study suggests that STAT5B should be considered as a candidate gene, and the variants identified as useful genetic markers for improved milk composition and udder health.
Keywords
dairy cattle; mastitis; milk performance; STAT5B; SNPs
Hrčak ID:
271523
URI
Publication date:
12.12.2021.
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