Veterinarska stanica, Vol. 54 No. 1, 2023.
Original scientific paper
https://doi.org/10.46419/vs.54.1.3
Total antioxidant status of bitches before and after ovariectomy
Tugomir Karadjole
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Ljerka Fučkala
; Ministry of Agriculture, Veterinary and Food Safety Administration, Zagreb
Ljiljana Bedrica
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Nino Maćešić
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Goran Bačić
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Ivan Folnožić
orcid.org/0000-0002-8988-0862
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Branimira Špoljarić
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Juraj Šavorić
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Ivan Butković
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Nikica Prvanović Babić
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Maša Efendić
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Miroslav Tomić
; Sisters of Charity Hospital, Zagreb, Croatia
Vesna Sokol Karadjole
; University Hospital Centre Zagreb, Croatia
Sandra Kunštek
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Martina Lojkić
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Abstract
Surgery impairs homeostasis, which is manifested in immunological, neuroendocrine, and metabolic changes in the organism. It is assumed that the response of the organism is proportional to the extent of the operative injury. Accordingly, the aim of this study was to determine the antioxidant status of bitches before and after ovariectomy. Fifteen bitches were presented to the Clinic for Reproduction and Obstetrics, Faculty of Veterinary Medicine, Zagreb, for elective ovariectomy due to castration. Blood sampling was performed on Day 0 (before surgery) and on Days 1, 3 and 7 after surgery to measure total antioxidant status (TAS), complete blood count, biochemistry and CRP. Total antioxidant capacity was determined using TAS test kits. For the purpose of standardisation of reference values of TAS in laboratory, 30 serum samples of healthy, intact bitches were also analysed and the mean value +/- two standard deviations was used as the reference value. The average reference value was 1.55 mmol/L. The values of total antioxidant capacity after surgery on all sampling days did not differ significantly. Comparing the minimum and maximum values on all sampling days, there were no statistical differences in the values of the total antioxidant capacity in ovariectomised bitches. Based on these results, it can be concluded that ovariectomy did not cause oxidative stress.
Keywords
ovariectomy; bitch; total antioxidant status
Hrčak ID:
273744
URI
Publication date:
25.5.2022.
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