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Veterinary Archives, Vol. 92 No. 4, 2022.

Original scientific paper

https://doi.org/10.24099/vet. arhiv.1300

Characterization of somatic and metabolic antigens of Cysticercus cellulosae and determination of immunodominant proteins

Kaushik Satyaprakash ; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India; Department of Veterinary Public Health and Epidemiology, Faculty of Veterinary and Animal Sciences, RGSC (Banaras Hindu University), Mirzapur, Uttar Pradesh, India
Wiqar Ahmed Khan ; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India
Nandkishor Namdeorao Zade ; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India
Sandeep Prabhakarrao Chaudhari ; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India
Shilpshri Vasant Shinde ; Department of Veterinary Public Health and Epidemiology, Nagpur Veterinary College, Nagpur, Maharashtra, India

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Abstract

The present study shows the characterization of four somatic antigens (Whole Cyst Antigen (WCA), Cystic Fluid Antigen (CFA), Scolex Antigen (SA) and Membrane-Body Antigen (MBA)) and one metabolic antigen (Excretory-Secretory Antigen (ESA)) prepared from Cysticercus cellulosae originated from a naturally infected pig. Immunodominat proteins were determined using hyper immune rabbit sera. The Sodium Dodecyl Sulphate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) profile of the antigens revealed different numbers and patterns of protein bands in the range of 11.80 to 176.74 kDa. The electrophoretic pattern of ESA also revealed a number of closely placed indistinct bands in the range of 20-34 kDa.. The proteins in the lower molecular weight range (< 30 kDa) in the case of WCA, CFA, ESA, and the medium molecular weight range (30-60 kDa) in the case of SA and MBA were observed to be immunodominant in the Enzyme-Linked Immunoelectrotransfer Blot (EITB) assay. These immunodominant proteins could be further investigated as potential diagnostic antigens in the serodiagnosis of cysticercosis in pigs, and neurocysticercosis in human patients.

Keywords

cysticercosis; pig; antigens; EITB; immunodominant proteins

Hrčak ID:

283891

URI

https://hrcak.srce.hr/283891

Publication date:

26.9.2022.

Article data in other languages: croatian

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